SYNTHETIC ANTIGENS AS IMMUNOGENS .4. SPECIFICITY OF ANTISERA DEVELOPED TO GAL BETA-1-3(GLCNAC BETA-1-6)GALNAC AND GLCNAC BETA-1-6GALNAC

Antisera to the chemically synthesized trisaccharide, Gal beta-1-3(GlcNAc beta-1-6)GalNAc, and disaccharide, GlcNAc beta-1-6GalNAc, were developed using the diazophenyl bovine serum albumin derivatives. The binding specificity of the antisera were analyzed by enzyme immunoassays with structurally related, chemically synthesized oligosaccharides. The anti-trisaccharide antibody showed no reactivity to T antigenic structures which bear the Gal beta-1-3GalNAc moiety. The immunodominant area of the Gal beta-1-3(GlcNAc beta-1-6)GalNAc was contained in the GlcNAc beta-1-6GalNAc region of the molecule. The reactivity pattern of the anti-trisaccharide antibody, although directed to the disaccharide, did not exactly duplicate the reactivity pattern of the anti-disaccharide.