SYNTHETIC ANTIGENS AS IMMUNOGENS .4. SPECIFICITY OF ANTISERA DEVELOPED TO GAL BETA-1-3(GLCNAC BETA-1-6)GALNAC AND GLCNAC BETA-1-6GALNAC

被引：3
作者：
MATTA, KL
PISKORZ, CF
ABBAS, SA
DIAKUN, KR
机构：
[1] Department of Gynecologic Oncology, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY
来源：
IMMUNOLOGICAL INVESTIGATIONS | 1991年 / 20卷 / 5-6期
关键词：
D O I：
10.3109/08820139109082633
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Antisera to the chemically synthesized trisaccharide, Gal beta-1-3(GlcNAc beta-1-6)GalNAc, and disaccharide, GlcNAc beta-1-6GalNAc, were developed using the diazophenyl bovine serum albumin derivatives. The binding specificity of the antisera were analyzed by enzyme immunoassays with structurally related, chemically synthesized oligosaccharides. The anti-trisaccharide antibody showed no reactivity to T antigenic structures which bear the Gal beta-1-3GalNAc moiety. The immunodominant area of the Gal beta-1-3(GlcNAc beta-1-6)GalNAc was contained in the GlcNAc beta-1-6GalNAc region of the molecule. The reactivity pattern of the anti-trisaccharide antibody, although directed to the disaccharide, did not exactly duplicate the reactivity pattern of the anti-disaccharide.
引用
收藏
页码：525 / 537
页数：13
相关论文
共 32 条
  • [1] SYNTHETIC STUDIES IN CARBOHYDRATES .25. SYNTHESIS OF BENZYL 2-ACETAMIDO-3-O AND 6-O-(2-ACETAMIDO-2-DEOXY-BETA-D-GLUCOPYRANOSYL)-2-DEOXY-ALPHA-D-GALACTOPYRANOSIDE
    ABBAS, SA
    BARLOW, JJ
    MATTA, KL
    [J]. CARBOHYDRATE RESEARCH, 1983, 112 (02) : 201 - 211
  • [2] BAST RC, 1987, CANCER-AM CANCER SOC, V60, P1984, DOI 10.1002/1097-0142(19901015)60:8+<1984::AID-CNCR2820601510>3.0.CO
  • [3] 2-W
  • [4] BASU M, 1987, METHOD ENZYMOL, V138, P575
  • [5] BHAVANANDAN VP, 1988, INDIAN J BIOCHEM BIO, V25, P36
  • [6] MUCIN SYNTHESIS .7. CONVERSION OF R1-BETA-1-3GAL-R2 TO R1-BETA-1-3(GLCNAC-BETA-1-6)GAL-R2 AND OF R1-BETA-1-3GALNAC-R2 TO R1-BETA-1-3(GLCNAC-BETA-1-6)GALNAC-R2 BY A BETA-6-N-ACETYLGLUCOSAMINYLTRANSFERASE IN PIG GASTRIC-MUCOSA
    BROCKHAUSEN, I
    MATTA, KL
    ORR, J
    SCHACHTER, H
    KOENDERMAN, AHL
    VANDENEIJNDEN, DH
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1986, 157 (03): : 463 - 474
  • [7] CHATTERJEE SK, 1979, CANCER RES, V39, P1943
  • [8] SYNTHETIC ANTIGENS AS IMMUNOGENS .1. THE COMBINING SITE SPECIFICITIES OF ANTIBODIES FORMED IN RABBITS TO SYNTHETIC DISACCHARIDE ALPHA-L-FUC-(1-]3)-D-GAL
    DIAKUN, KR
    YAZAWA, S
    VALENZUELA, L
    ABBAS, SA
    MATTA, KL
    [J]. IMMUNOLOGICAL INVESTIGATIONS, 1987, 16 (01) : 1 - 11
  • [9] DIAKUN KR, 1989, J IMMUNOL, V142, P2037
  • [10] SYNTHETIC ANTIGENS AS IMMUNOGENS .2. ANTIBODIES TO SYNTHETIC T-ANTIGEN
    DIAKUN, KR
    YAZAWA, S
    VALENZUELA, L
    ABBAS, SA
    MATTA, KL
    [J]. IMMUNOLOGICAL INVESTIGATIONS, 1987, 16 (02) : 151 - 163
1234