FLUOROMETRIC ASSAY OF PYRIDOXAL AND PYRIDOXAL 5'-PHOSPHATE

被引：17

作者：

CHAUHAN, MS

DAKSHINAMURTI, K

机构：

[1] Department of Biochemistry, Faculty of Medicine, University of Manitoba, Winnipeg

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 96卷 / 02期

关键词：

D O I：

10.1016/0003-2697(79)90603-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new and very sensitive fluorometric method for the determination of pyridoxal and pyridoxal 5′-phosphate is reported. The specificity is based on the reductive amination of pyridoxal and its 5′-phosphate with methyl anthranilate and sodium cyanoborohydride at pH 4,5 to 5,0. Separation of the highly fluorescent methyl-N-pyridoxyl anthranilate was achieved by a combination of column and thin-layer chromatography on silica gel. This method has been applied to the assay of pyridoxal and pyridoxal 5′-phosphate in seruum. © 1979.

引用

页码：426 / 432

页数：7

共 18 条

[1] MEASUREMENT OF SERUM PYRIDOXAL BY A MICROBIOLOGICAL ASSY USING LACTOBACILLUS-CASEI [J].