GENETIC-CHARACTERIZATION OF OMPH MUTANTS IN THE DEEP-SEA BACTERIUM PHOTOBACTERIUM SP STRAIN SS9

被引:3
作者
BARTLETT, D
CHI, E
机构
关键词
OUTER MEMBRANE PROTEIN; PORIN; EXTREMOPHILE; PIEZOPHILE; BAROPHILE; HIGH PRESSURE ADAPTION; DEEP-SEA BACTERIUM; PHOTOBACTERIUM; GENE REPLACEMENT; TRANSPOSON;
D O I
10.1007/s002030050145
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
OmpH is an outer membrane protein produced by the deep-sea bacterium Photobacterium species strain SS9 in response to elevated hydrostatic pressure. In order to facilitate studies of the function of this protein, a series of OmpH(+) and OmpH(-) strains were obtained from SS9 by Tn5 gene replacement mutagenesis. A previously isolated ompH::lacZ strain and a derivative of this strain harboring a plasmid expressing the wild-type ompH gene were also utilized. The acridine mutagen ICR 191 preferentially inhibited the growth of OmpH(+) over OmpH(-) cells. Indeed, OmpH(+) cultures treated with the mutagen rapidly accumulated mutants producing reduced levels of OmpH. In addition, OmpH(+) cells took up the peptide Met-Leu-Phe approximately 15 times more rapidly than OmpH(-) cells. The results are consistent with the hypothesis that OmpH functions as a relatively large, nonspecific diffusion channel.
引用
收藏
页码:323 / 328
页数:6
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