PERFUSION CULTURE APPARATUS FOR SUSPENDED MAMMALIAN-CELLS

被引：20

作者：

TOKASHIKI, M

TAKAMATSU, H

机构：

[1] Tokyo Research Center, Teijin Limited, Hino-shi, Tokyo, 191

来源：

CYTOTECHNOLOGY | 1993年 / 13卷 / 03期

关键词：

ANCHORAGE INDEPENDENT MAMMALIAN CELLS; CENTRIFUGATION; FILTRATION; GRAVITATIONAL SETTLING; HIGH DENSITY PERFUSION CULTURE; SUSPENDED-CELL CULTURE;

D O I：

10.1007/BF00749811

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A variety of processes have been proposed for mammalian cell culture in the commercial production of useful substances (e.g., monoclonal antibodies, therapeutic and diagnostic proteins). Among them, the perfusion culture of suspended non-immobilized cells is the most advantageous. Perfusion culture can be classified by the separation process of suspended cells from the culture mixture into three types, namely filtration, gravitational settling and centrifugation. From a commercial point of view, the present situation and technical problems of suspended-cell perfusion culture will be reviewed based on the three types, The recent development of perfusion culture has been carried out mainly on the filtration separation process, but the centrifugation process seems to have a promising future because of operation stability and scale-up feasibility. The reasons will be explained in details.

引用

页码：149 / 159

页数：11

共 44 条

[1]

APELMAN S, 1992, ANIMAL CELL TECHNOLOGY : BASIC & APPLIED ASPECTS, VOL 4, P149

[2]

ARAI T, 1993, ANIMAL CELL TECHNOLO, P341

[3] SPIN FILTER PERFUSION SYSTEM FOR HIGH-DENSITY CELL-CULTURE - PRODUCTION OF RECOMBINANT URINARY TYPE PLASMINOGEN-ACTIVATOR IN CHO CELLS [J].