DIFFERENTIALLY EXPRESSED MESSENGER-RNAS AS A CONSEQUENCE OF OXIDATIVE STRESS IN INTACT-CELLS

Intracellular redox conditions influence the activity of several transcription factors leading to a modulation of the expression of the genes controlled by these factors, We examined the changes in cell transcription patterns after oxidative stress induced by diethylmaleate (DEM), Using the differential display technique me identified several differentially expressed sequence tags, four of which are identical or highly homologous to sequences contained in the human cDNAs encoding vimentin, c-fos, cytochrome oxidase IV and ribosomal protein L4; another one corresponds to a transcript of the mitochondrial genome of unknown function, The remaining five cDNAs are not recorded in any sequence data bank. One of these, named Rox3, lights up two mRNA species of similar to 3400 and 3600 bp, significantly increased after treatment,vith DEM or with other oxidizing agents, This increase appears precociously after exposure to DEM and it is completely prevented by pretreatment with N-acetylcysteine. The Rox3 fragment was used to screen a cDNA library; one fully sequenced clone showed 100% homology with the putative human guanine nucleotide regulatory protein nep1.