A SIMPLE ION-EXCHANGE CHROMATOGRAPHIC DETERMINATION OF NONVOLATILE ORGANIC-ACIDS IN SOME SPANISH EXOTIC FRUITS

被引：21

作者：

CANO, MP ^{[1
]}

TORIJA, E ^{[1
]}

MARIN, MA ^{[1
]}

CAMARA, M ^{[1
]}

机构：

[1] UNIV COMPLUTENSE MADRID,DEPT NUTR & FOOD SCI 2,E-28040 MADRID,SPAIN

来源：

ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG | 1994年 / 199卷 / 03期

关键词：

D O I：

10.1007/BF01193447

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

High-performance liquid chromatography (HPLC) permits accurate determination of non-volatile acids in fruit. The Spanish exotic fruits assayed were kiwi fruits (Actinidia chinensis), cvs Hayward, Bruno, Monty and Abbot; mangoes (Mangifera indica), cvs Smith and Lippens; papayas (Carica papaya), cv Solo; pineapples (Ananus comosus), cv Smooth Cayenne; and bananas (Musa cavendishii), cv Enana. Sample preparation for HPLC analysis basically consisted of methanol extraction, followed by an easy clean-up with a C18 cartridge. Separation of the different acids was accomplished using an ion-exchange column and detection was monitored at 214 nm. Reproducibility of the minor organic acids was quite acceptable, and the procedure described offers a rapid analytical alternative to gas chromatography. Oxalic, oxoglutaric, citric, galacturonic, tartaric, ascorbic, L-malic, quinic, succinic, fumaric and D-malic acids can be quantified by this method.

引用

页码：214 / 218

页数：5

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