A SIMPLE ION-EXCHANGE CHROMATOGRAPHIC DETERMINATION OF NONVOLATILE ORGANIC-ACIDS IN SOME SPANISH EXOTIC FRUITS

被引:21
作者
CANO, MP [1 ]
TORIJA, E [1 ]
MARIN, MA [1 ]
CAMARA, M [1 ]
机构
[1] UNIV COMPLUTENSE MADRID,DEPT NUTR & FOOD SCI 2,E-28040 MADRID,SPAIN
来源
ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG | 1994年 / 199卷 / 03期
关键词
D O I
10.1007/BF01193447
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
High-performance liquid chromatography (HPLC) permits accurate determination of non-volatile acids in fruit. The Spanish exotic fruits assayed were kiwi fruits (Actinidia chinensis), cvs Hayward, Bruno, Monty and Abbot; mangoes (Mangifera indica), cvs Smith and Lippens; papayas (Carica papaya), cv Solo; pineapples (Ananus comosus), cv Smooth Cayenne; and bananas (Musa cavendishii), cv Enana. Sample preparation for HPLC analysis basically consisted of methanol extraction, followed by an easy clean-up with a C18 cartridge. Separation of the different acids was accomplished using an ion-exchange column and detection was monitored at 214 nm. Reproducibility of the minor organic acids was quite acceptable, and the procedure described offers a rapid analytical alternative to gas chromatography. Oxalic, oxoglutaric, citric, galacturonic, tartaric, ascorbic, L-malic, quinic, succinic, fumaric and D-malic acids can be quantified by this method.
引用
收藏
页码:214 / 218
页数:5
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