Label Free Detection of L-Glutamate Using Microfluidic Based Thermal Biosensor

A thermoelectric biosensor for the detection of L-glutamate concentration was developed. The thermoelectric sensor is integrated into a micro-calorimeter which measures the heat produced by biochemical reactions. The device contains a single flow channel that is 120 mu m high and 10 mm wide with two fluid inlets and one fluid outlet. An antimony-bismuth (Sb-Bi) thermopile with high common mode rejection ratio is attached to the lower channel wall and measures the dynamic changes in the temperature when L-glutamate undergoes oxidative deamination in the presence of glutamate oxidase (GLOD). The thermopile has a Seebeck coefficient of similar to 7 mu V(m center dot <bold>K</bold>)(-1). The device geometry, together with hydrodynamic focusing, eliminates the need of extensive temperature control. Layer-by-layer assembly is used to immobilize GLOD on the surface of glass coverslips by alternate electrostatic adsorption of polyelectrolyte and GLOD. The impulse injection mode using a 6-port injection valve minimizes sample volume to 5 mu L. The sensitivity of the sensor for glutamate is 17.9 nVsm center dot M-1 in the linear range of 0-54 mM with an R-2 value of 0.9873. The lowest detection limit of the sensor for glutamate is 5.3 mM.