DETECTION OF MYCOBACTERIUM-PARATUBERCULOSIS BY POLYMERASE CHAIN-REACTION IN CHILDREN WITH CROHNS-DISEASE

被引：110
作者：
DELLISOLA, B
POYART, C
GOULET, O
MOUGENOT, JF
SADOUNJOURNO, E
BROUSSE, N
SCHMITZ, J
RICOUR, C
BERCHE, P
机构：
[1] HOP NECKER ENFANTS MALAD,SERV GASTROINTEROL PEDIAT,MICROBIOL LAB,F-75050 PARIS,FRANCE
[2] HOP NECKER ENFANTS MALAD,SERV GASTROENTEROL PEDIAT,ANATOMOPATHOL LAB,PARIS,FRANCE
[3] HOP NECKER ENFANTS MALAD,DEPT PEDIAT,PARIS,FRANCE
来源：
JOURNAL OF INFECTIOUS DISEASES | 1994年 / 169卷 / 02期
关键词：
D O I：
10.1093/infdis/169.2.449
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Mycobacterium paratuberculosis, the causative agent of Johne's disease (a chronic enteritis in ruminants), has been suspected to be involved in Crohn's disease. In this study, polymerase chain reaction was used to detect the presence of IS900 DNA sequences specific to M. paratuberculosis genomes in biopsies and surgical resections from 53 children with various gastrointestinal diseases and disorders. IS900 sequences were found in 13 of 18 samples from patients with Crohn's disease (72%; P < .01 vs. samples from patients without Crohn's disease), in 1 of 5 with ulcerative colitis, in 2 of 6 with severe unclassified colitis, and in 7 of 24 with other gastrointestinal illnesses. These results appear to support the hypothesis that M. paratuberculosis is involved in the pathogenesis of Crohn's disease.
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页码：449 / 451
页数：3
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