HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE ANALYSIS OF AZADIRACHTIN IN 3 COMMERCIAL FORMULATIONS AND NEEM OIL

被引：20
作者：
SUNDARAM, KMS
CURRY, J
机构：
[1] Forestry Canada, Forest Pest Management Institute, Sault Ste. Marie, Ontario, P6A 5M7, P.O. Box 490
来源：
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES | 1993年 / 28卷 / 02期
关键词：
NATURAL PRODUCTS; AZADIRACHTIN; HPLC ANALYSIS; NEEM FORMULATIONS; NEEM OIL;
D O I：
10.1080/03601239309372824
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
An improved high performance liquid chromatographic method is reported to determine azadirachtin (AZ) content in neem formulations and neem oil. Aqueous methanol solutions of samples were extracted sequentially with hexane and dichloromethane. The dichloromethane layer, containing AZ, was taken to dryness and reconstituted in ethyl acetate. It was purified by Florisil(R) column cleanup, eluted with ethyl acetate and injected into the liquid chromatograph. Separation was performed with a reversed-phase Spherisorb C-18 ODS 5 pm column with an acetonitrile/water gradient system. The eluent was monitored with UV detection at 210 nm. The method was found to be sufficiently rapid and reproducible for use in the analysis of AZ in neem formulations and neem oil. Limits of quantification and detection were respectively 6 mug/g and 3 mug/g.
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页码：221 / 241
页数：21
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