TEMPORAL CHANGES IN INTRACELLULAR-DISTRIBUTION OF PROTEIN-KINASE-C IN SWISS 3T3 CELLS DURING MITOGENIC STIMULATION WITH INSULIN-LIKE GROWTH FACTOR-I AND BOMBESIN - TRANSLOCATION TO THE NUCLEUS FOLLOWS RAPID CHANGES IN NUCLEAR POLYPHOSPHOINOSITIDES

Using a polyclonal antibody raised against a synthetic peptide of the catalytic region of protein kinase C, we have carried out a combined immunocytochemical and immunochemical analysis to follow the subcellular localisation of this enzyme in response to mitogenic stimulation with insulin-like growth factor I and bombesin. These investigations show a time dependent translocation of protein kinase C from the cytoplasm to the nucleus since 5 min stimulation reaching a maximal effect after 45 min. These results show clearly that mitogen induced translocation of protein kinase C to the nucleus follows temporally the earlier changes in nuclear polyphosphoinositide metabolism previously demonstrated. © 1991.