ACTIVATION OF HEART SARCOLEMMAL CA2+-MG2+ ATPASE BY CYCLIC AMP-DEPENDENT PROTEIN-KINASE

被引：31
作者：
ZIEGELHOFFER, A
ANANDSRIVASTAVA, MB
KHANDELWAL, RL
DHALLA, NS
机构：
[1] UNIV MANITOBA,DEPT PHYSIOL,DEPT EXPTL CARDIOL,WINNIPEG R3E 0W3,MANITOBA,CANADA
[2] UNIV MANITOBA,DEPT ORAL BIOL,WINNIPEG R3E 0W3,MANITOBA,CANADA
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 89卷 / 04期
关键词：
D O I：
10.1016/0006-291X(79)92117-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The sarcolemmal membrane obtained from rat heart by hypotonic shock-LiBr treatment method was found to incorporate 32P from [γ-32P] ATP in the absence and presence of cyclic AMP and protein kinase. The phosphorylated membrane showed an increase in Ca2+ ATPase and Mg2+ ATPase activities without any changes in Na+K+ ATPase activity. The observed increase in Ca2+ Mg2+ ATPase activity was found to be associated with an increase in Vmax value of the reaction whereas Ka value for Ca2+ Mg2+ was not altered. These results provide information concerning biochemical mechanism for increased calcium entry due to hormones which are known to elevate cyclic AMP levels in myocardium and produce a positive inotropic effect. © 1979.
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页码：1073 / 1081
页数：9
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