RADIOIMMUNOASSAY OF AN ANTIBODY TO PHI-X174 DNA-RNA HYBRID

被引：13
作者：
NAKAZATO, H
机构：
[1] Laboratory of Tumor Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda
来源：
ANALYTICAL BIOCHEMISTRY | 1979年 / 98卷 / 01期
关键词：
D O I：
10.1016/0003-2697(79)90707-3
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A fast and simple radioimmunoassay (RIA) technique was developed for an antibody to DNA·RNA hybrid using protein A-bearing Staphylococcus aureus cells as immuno-adsorbent and a glass microfiber filter for the deparation of free antigen and antibody-antigen complex. A simple method for preparing 3H-labeled DNA·RNA hybrid using single-stranded circular DNA of viruses and Escherichia coli RNA polymerase (nucleosidetri-phosphate: RNA nucleotidyltransferase, EC 2.7.7.6) is also described. In comparison to a hybrid made of natural sequences, a synthetic homopolymer hybrid poly (A)·poly(dT) was found to be a poor competitor for the antibody by this RIA technique. © 1979.
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页码：74 / 80
页数：7
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