ADENOSINE-INDUCED CYCLIC-AMP INCREASE IN PIG LYMPHOCYTES IS NOT RELATED TO ADENYLATE-CYCLASE STIMULATION

被引：25
作者：
BONNAFOUS, JC
DORNAND, J
MANI, JC
机构：
[1] Laboratoire de Biochimie des Membranes, ER CNRS No. 228, Ecole Nationale Supérieure de Chimie, Montpellier
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 587卷 / 02期
关键词：
(Lymphocyte); Adenosine; Adenylate cyclase; Concanavalin A; Cyclic AMP increase; Phosphodiesterase;
D O I：
10.1016/0304-4165(79)90352-0
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Adenosine-cyclic AMP relationships have been studied in pig mesenteric lymph node lymphocytes. The early 2-3-fold increase in cyclic AMP accumulation elicited by adenosine and 2-chloroadenosine, an adenosine deaminase-resistant analogue, could not be correlated to similar effects on the adenylate cyclase activity of disrupted cell preparations, but rather to the competitive inhibition of the low Km (0.17 μM) cyclic AMP phosphodiesterase. The existence of adenosine receptors coupled to lymphocyte adenylate cyclase, which had been proposed by several authors, could not be confirmed by this study. Adenosine-cyclic AMP relationships do not appear to be involved in concanavalin A stimulation of pig lymphocytes. © 1979.
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页码：180 / 191
页数：12
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