IMAGING SPECTROMETERS FOR FLUORESCENCE AND RAMAN MICROSCOPY - ACOUSTOOPTIC AND LIQUID-CRYSTAL TUNABLE FILTERS

被引:224
作者
MORRIS, HR
HOYT, CC
TREADO, PJ
机构
[1] UNIV PITTSBURGH, DEPT CHEM, PITTSBURGH, PA 15260 USA
[2] CAMBRIDGE RES & INSTRUMENTAT INC, CAMBRIDGE, MA 02139 USA
关键词
SPECTRAL IMAGING; IMMUNOFLUORESCENCE MICROSCOPY; ACOUSTOOPTIC TUNABLE FILTER (AOTF); LIQUID CRYSTAL TUNABLE FILTER (LCTF); LYOT FILTER; POLYSTYRENE; MICROSPHERES; NEUROTRANSMITTERS; SEROTONIN (5HT); RETICULOSPINAL NEURONS; RAMAN MICROSCOPY;
D O I
10.1366/0003702944029820
中图分类号
TH7 [仪器、仪表];
学科分类号
0804 ; 080401 ; 081102 ;
摘要
Acousto-optic tunable filters (AOTF) and liquid crystal tunable filters (LCTF) are evaluated for their suitability as fluorescence microscopy imaging spectrometers. AOTFs are solid-state birefringent crystals that provide an electronically tunable spectral notch passband in response to an applied acoustic field. LCTFs also provide a notch passband that can be controlled by incorporating liquid crystal waveplate retarders within a Lyot birefringent filter. In this paper, spectroscopic performance and imaging quality are contrasted by evaluation of model systems. Studies include transmission imaging of standard resolution targets, multispectral fluorescence emission imaging of tagged polystyrene microspheres, and immunofluorescence imaging of neurotransmitters within rat-brain-stem thin sections. In addition, the first use of LCTFs for Raman microscopy is demonstrated. Raman microscopy is a noninvasive spectral imaging technique that can provide chemically significant image contrast complementary to fluorescence microscopy without the use of stains or tags.
引用
收藏
页码:857 / 866
页数:10
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