GENERATION OF AN ANTIBODY TO HLA-G IN TRANSGENIC MICE AND DEMONSTRATION OF THE TISSUE REACTIVITY OF THIS ANTIBODY

被引：46

作者：

CHUMBLEY, G

KING, A

GARDNER, L

HOWLETT, S

HOMLES, N

LOKE, YW

机构：

[1] UNIV CAMBRIDGE,DEPT PATHOL,HUMAN REPROD IMMUNOBIOL RES GRP,CAMBRIDGE CB2 1QP,ENGLAND

[2] UNIV CAMBRIDGE,DEPT PATHOL,DIV IMMUNOL,CAMBRIDGE CB2 1QP,ENGLAND

来源：

JOURNAL OF REPRODUCTIVE IMMUNOLOGY | 1994年 / 27卷 / 03期

基金：

英国惠康基金;

关键词：

HLA-G ANTIBODY; HLA-G TRANSFECTANTS; TRANSGENIC MICE; EXTRAVILLOUS TROPHOBLAST;

D O I：

10.1016/0165-0378(94)90002-7

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A method was devised to generate antibodies against the non-classical class I HLA-G antigen. This consisted of immunising HLA-A2/beta(2)m double transgenic mice with HLA-G transfected into mouse Ltk(-) cells. A polyclonal antibody was obtained which appears to be specific for HLA-G. The staining pattern of this antibody was restricted solely to all populations of extravillous trophoblast. No fetal tissues reacted with this antibody, including those where HLA-G mRNA has been demonstrated, such as fetal eye, thymus and liver. This study confirms that HLA-G is a trophoblast-specific protein, although it remains a possibility that the technique of immunohistology is not sufficiently sensitive to detect low level HLA-G antigen expression in non-trophoblast tissues.

引用

页码：173 / 186

页数：14