PRELIMINARY-X-RAY DIFFRACTION STUDY OF A NEW CRYSTAL FORM OF C-1027-AG, THE APOPROTEIN OF THE MACROMOLECULAR ANTITUMOR ANTIBIOTIC C-1027 FROM STREPTOMYCES-GLOBISPORUS

被引：1
作者：
MOTOJIMA, F
INAKA, K
MINAMI, Y
OTANI, T
MIKI, K
机构：
[1] KYOTO UNIV,FAC SCI,DEPT CHEM,SAKYO KU,KYOTO 60601,JAPAN
[2] TOKYO INST TECHNOL,RESOURCES UTILIZAT RES LAB,MIDORI KU,YOKOHAMA 226,KANAGAWA,JAPAN
[3] TAISHO PHARMACEUT CO LTD,TOKUSHIMA RES CTR,TOKUSHIMA 77101,JAPAN
来源：
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1995年 / 51卷
关键词：
D O I：
10.1107/S0907444995004197
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A new crystal form of C-1027-AG, the apoprotein of the macromolecular antitumor antibiotic C-1027 isolated from Streptomyces globisporus was obtained by the vapor-diffusion procedure using lithium sulfate as a precipitant. In the present crystallization, it is noteworthy that targe-sized single crystals successfully grew from very small droplets (less than 1.0 mu l). The present crystals belong to the trigonal system, space group P3(1)21 or P3(2)21 with cell dimensions of a = b = 62.6 and c = 54.2 Angstrom. Assuming that the asymmetric unit contains one molecule, the V-m value is calculated as 2.9 Angstrom(3) Da(-1). A total of 3654 independent reflections from two native crystals was obtained up to 2.5 Angstrom resolution with synchrotron radiation, the merging R factor being 0.097.
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页码：1084 / 1085
页数：2
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