PURIFICATION OF STAPHYLOCOCCAL-ENTEROTOXIN A BY DYE-LIGAND CHROMATOGRAPHY

被引：0

作者：

LOPES, HR ^{[1
]}

MILHOMEM, AM ^{[1
]}

NOLETO, ALS ^{[1
]}

BERGDOLL, MS ^{[1
]}

机构：

[1] UFRJ,INST MICROBIOL,CTR CIENCIAS SAUDE,BLOCO 1,CIDADE UNIV,ILHA FUNDAO,BR-21941 RIO JANEIRO,BRAZIL

来源：

REVISTA DE MICROBIOLOGIA | 1993年 / 24卷 / 04期

关键词：

STAPHYLOCOCCAL ENTEROTOXIN-A; PURIFICATION; CHROMATOGRAPHY;

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Enterotoxin A was purified from 400 ml of Staphylococcus aureus strain FRI-722 culture supernatant fluid. The sac culture method was used to produce the enterotoxin. Ten milligrams (36%) of purified enterotoxin A was obtained from the 28 mg present in the culture supernatant fluid. The purified enterotoxin was homogeneous when analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The procedures used are adequate for purifying a satisfactory amount of enterotoxin and can be used in laboratories where the necessary equipment is not available for handling large volumes of culture supernatant fluid.

引用

页码：228 / 231

页数：4