THE MUTANT ASN(291)-]SER HUMAN LIPOPROTEIN-LIPASE IS ASSOCIATED WITH REDUCED CATALYTIC ACTIVITY AND DOES NOT INFLUENCE BINDING TO HEPARIN

Lipoprotein lipase (LPL) plays a central role in triglyceride metabolism, regulating the catabolism of triglyceride-rich lipoprotein particles, LPL performs its hydrolytic action attached to heparan sulfate proteoglycans at the luminal surface of capillary endothelial cells, We have assessed the effect of the Asn(291) --> Ser (N291S) substitution found in LPL gene from a human hyperlipemic patient, Our results showed that both the wild-type (WT) and N291S hLPL expressed in COS1 cells were secreted to the extracellular medium, and presented similar intracellular distibution patterns, Furthermore, heparin-Sepharose affinity chromatography assays revealed normal heparin affinity of the N291S hLPL, In addition, both the mutant and the WT protein bound to the surface of human fibroblasts and untransfected COS1 cells, Interestingly, disminished LPL specific activity was observed in the extracellular medium from mutant expressing cells, Therefore the lack of normal LPL activity in patients harbouring such a mutation could be the cause of their hyperlipemic disorder.