Aberrant renal expression of peroxisome coactivator PGC-1 alpha and its regulators (Sirt1 & GSK3 beta) in rats with diabetic nephropathy

被引：0

作者：

Mohamad, Magda I. ^{[1
]}

Aboelhussein, Marwa M. ^{[1
]}

Elayat, Wael M. ^{[1
]}

Elshormilisy, Amr Aly ^{[2
]}

机构：

[1] Ain Shams Univ, Dept Med Biochem, Fac Med, Cairo, Egypt

[2] Helwan Univ, Dept Internal Med, Fac Med, Helwan, Egypt

来源：

GENE REPORTS | 2018年 / 13卷

关键词：

PGC-1; alpha; Sirt-1; GSK3; beta; Diabetic nephropathy;

D O I：

10.1016/j.genrep.2018.03.007

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The dysregulation of peroxisome proliferator-activated receptor-gamma coactivator-1 alpha (PGC-1 alpha) has been implicated in diabetes mellitus. However, the role of PGC-1 alpha in diabetic nephropathy (DN) is still inconclusive. Our aim was to evaluate the renal expression of PGC-1 alpha and its regulators: silent information regulator 2 homolog (Sirt-1) and glycogen synthase kinase3 beta (GSK3 beta) in the diabetic rats. Nineteen adult male rats were randomly divided into the control group (n = 6) and the diabetic group (n. = 13). The expression of these genes was performed by quantitative real-time PCR (qRT-PCR) in the renal tissues. Also, catalase activity was measured by a colorimetric method. The diabetic rats had higher expression levels of PGC-1 alpha and GSK3 beta; however, the expression of Sirt-1 and catalase activity was significantly lower in comparison to the control group (p < 0.05). We concluded that the aberrant expression of PGC-1 alpha, Sirt-1, and GSK3 beta may be associated with the developing of DN.

引用

页码：229 / 233

页数：5

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