THE EFFECT OF DOXORUBICIN ON THE GLUTATHIONE CONTENT AND VIABILITY OF CULTURED HUMAN LUNG-CANCER CELL-LINES A549 AND GLC4210

被引：6
作者：
ALKABBAN, M
STEWART, MJ
WATSON, ID
REGLINSKI, J
机构：
[1] GLASGOW ROYAL INFIRM,INST CLIN BIOCHEM,GLASGOW G4 0SF,SCOTLAND
[2] UNIV STRATHCLYDE,DEPT PURE & APPL CHEM,GLASGOW G1 1XW,SCOTLAND
来源：
CLINICA CHIMICA ACTA | 1990年 / 194卷 / 2-3期
关键词：
LUNG TUMORS; DOXORUBICIN; GLUTATHIONE; HPLC;
D O I：
10.1016/0009-8981(90)90127-E
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Glutathione was measured in doxorubicin-sensitive cells from small cell carcinoma of lung (GLC4 210), and the levels compared with those of cells with acquired resistance and a line of resistant non-small-cell adenocarcinoma A549 (Alveolar type 2). The effect of different doxorubicin concentrations on glutathione were measured by HPLC. The effect of doxorubicin on the viability of the cell lines was studied using thiazole blue dye reduction. An increase in A549 sensitivity to doxorubicin was produced using buthionine-S,R-sulfoximine at a non-toxic dose.
引用
收藏
页码：121 / 130
页数：10
相关论文
共 20 条
[1]   THE USE OF H-1 SPIN-ECHO NMR AND HPLC TO CONFIRM DOXORUBICIN INDUCED DEPLETION OF GLUTATHIONE IN THE INTACT HELA-CELL [J].
ALKABBAN, M ;
WATSON, ID ;
STEWART, MJ ;
REGLINSKI, J ;
SMITH, WE ;
SUCKLING, CJ .
BRITISH JOURNAL OF CANCER, 1988, 57 (06) :553-558
[2]  
ARRICK BA, 1984, CANCER RES, V44, P4224
[3]  
ARRICK BA, 1982, J BIOL CHEM, V257, P1231
[4]  
CROOK TR, 1986, CANCER RES, V46, P5035
[5]   L1210(A) MOUSE LYMPHOMA-CELLS DEPLETED OF GLUTATHIONE WITH L-BUTHIONINE-S-R-SULFOXIMINE PROLIFERATE IN TISSUE-CULTURE [J].
GAETJENS, EC ;
CHEN, P ;
BROOME, JD .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1984, 123 (02) :626-632
[6]   ALTERATIONS IN CELL-SURFACE MEMBRANES IN CHINESE-HAMSTER LUNG-CELLS RESISTANT TO ADRIAMYCIN [J].
GARMAN, D ;
CENTER, MS .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1982, 105 (01) :157-163
[7]   AUGMENTATION OF ADRIAMYCIN, MELPHALAN, AND CISPLATIN CYTO-TOXICITY IN DRUG-RESISTANT AND DRUG-SENSITIVE HUMAN OVARIAN-CARCINOMA CELL-LINES BY BUTHIONINE SULFOXIMINE MEDIATED GLUTATHIONE DEPLETION [J].
HAMILTON, TC ;
WINKER, MA ;
LOUIE, KG ;
BATIST, G ;
BEHRENS, BC ;
TSURUO, T ;
GROTZINGER, KR ;
MCKOY, WM ;
YOUNG, RC ;
OZOLS, RF .
BIOCHEMICAL PHARMACOLOGY, 1985, 34 (14) :2583-2586
[8]   EFFECTS OF GLUTATHIONE DEPLETION ON THE CYTOTOXICITY OF AGENTS TOWARD A HUMAN COLONIC TUMOR-CELL LINE [J].
JORDAN, J ;
DOHERTY, MD ;
COHEN, GM .
BRITISH JOURNAL OF CANCER, 1987, 55 (06) :627-631
[9]   DEPLETION OF TUMOR VERSUS NORMAL TISSUE GLUTATHIONE BY BUTHIONINE SULFOXIMINE [J].
LEE, FYF ;
ALLALUNISTURNER, MJ ;
SIEMANN, DW .
BRITISH JOURNAL OF CANCER, 1987, 56 (01) :33-38
[10]   ADRIAMYCIN ACCUMULATION AND METABOLISM IN ADRIAMYCIN-SENSITIVE AND ADRIAMYCIN-RESISTANT HUMAN OVARIAN-CANCER CELL-LINES [J].
LOUIE, KG ;
HAMILTON, TC ;
WINKER, MA ;
BEHRENS, BC ;
TSURUO, T ;
KLECKER, RW ;
MCKOY, WM ;
GROTZINGER, KR ;
MYERS, CE ;
YOUNG, RC ;
OZOLS, RF .
BIOCHEMICAL PHARMACOLOGY, 1986, 35 (03) :467-472
12