EFFECT OF ESTRADIOL BENZOATE ON HEPATIC-FUNCTION AND METABOLISM OF BILIARY CHOLESTEROL AND BILE-ACID IN QUAIL

Immature female quails were treated for 6 days with estradiol benzoate at daily 0.01-, 0.02-, 0.1-, and 1-mg dosages. At the end of treatment, bile outflow, bile cholesterol (CST), and bile acid (BA) secretory rates and liver, bile and serum CST and BA levels were determined. Some quails were used to measure the ratio (R) of the rates of intravenously injected [2-14C]acetate radioactivity incorporation in cholic (C) and chenodeoxycholic (CDC) acids excreted in bile. The oestrogenic treatments at doses greater than 0.01 mg/day caused a marked disturbance in hepatic function and in CST and BA metabolism: they induced an increase in relative liver weight, liver CST stores, serum CST, C, CDC and SGOT levels and in choleresis (respectively up to 56, 57, 650, 6000, 700, 42 and 235% increase at the daily 0.1-mg dosage) and they decreased bile total BA and CDC levels, bile and serum CDC to C level ratios, and R ratio (by 71, 81, 69, 84, and 58% respectively). An increase in the bile salts independent fraction of bile was responsible for hypercholeresis, whether alone at low dosage or in conjunction with other factors at higher dosage. These results are compared with those obtained in mammals, particularly in the rat.