IMMUNOHISTOCHEMICAL CHARACTERIZATION OF MONOCLONAL-ANTIBODIES DIRECTED AGAINST THE TSH RECEPTOR

被引：0

作者：

KOHNERT, KD ^{[1
]}

KRABBE, S ^{[1
]}

MENG, W ^{[1
]}

机构：

[1] UNIV GREIFSWALD, INTERNAL MED CLIN, DEPT ENDOCRINOL, O-2200 GREIFSWALD, GERMANY

来源：

ACTA HISTOCHEMICA | 1994年 / 96卷 / 02期

关键词：

MONOCLONAL ANTIBODIES; SPECIFICITY; TSH RECEPTOR; ELISA-IMMUNOPEROXIDASE;

D O I：

10.1016/S0065-1281(11)80174-1

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Monoclonal antibodies have been obtained by fusing mouse myeloma cells with spleen cells of mice immunized with crude thyroid membranes. Among the antibodies reactive with different thyroid antigenic components, three were found to specifically react with TSH receptor molecules. These antibodies displayed characteristic staining patterns on frozen sections of thyroid tissue from patients with various thyroid diseases upon identification of antibody binding by indirect peroxidase staining. No specific reactivity was detected with tissue from other human organs, such as pancreas, liver, fat, and muscle. The results demonstrate that the immunoperoxidase technique and the specificity of the monoclonal antibodies produced permitted the identification of cellular constituents that might be important antigens in autoimmune thyroid disease.

引用

页码：175 / 180

页数：6

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