VISUALIZATION OF CIRCULAR DNA-MOLECULES LABELED WITH COLLOIDAL GOLD SPHERES USING ATOMIC-FORCE MICROSCOPY

被引：24

作者：

SHAIU, WL ^{[1
]}

VESENKA, J ^{[1
]}

JONDLE, D ^{[1
]}

HENDERSON, E ^{[1
]}

LARSON, DD ^{[1
]}

机构：

[1] IOWA STATE UNIV SCI & TECHNOL,DEPT ZOOL & GENET,SIGNAL TRANSDUCT TRAINING GRP,AMES,IA 50011

来源：

JOURNAL OF VACUUM SCIENCE & TECHNOLOGY A-VACUUM SURFACES AND FILMS | 1993年 / 11卷 / 04期

关键词：

D O I：

10.1116/1.578311

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

We have imaged gold-labeled DNA molecules with the atomic force microscope (AFM). Circular plasmid DNA was labeled at internal positions by nick-translation using biotinylated dUTP. For visualization, the biotinylated DNA was linked to streptavidin-coated colloidal gold spheres (nominally 5 nm diam) prior to AFM imaging. Reproducible images of the labeled DNA were obtained both in dry air and under propanol. Height measurements of the DNA and colloidal gold made under both conditions are presented. The stability of the DNA-streptavidin colloidal gold complexes observed even under propanol suggests that this labeling procedure could be exploited to map regions of interest in chromosomal DNA.

引用

页码：820 / 823

页数：4

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