CLONING AND EXPRESSION OF A COMPLEMENTARY-DNA ENCODING A HIGH-AFFINITY HUMAN NEUROTENSIN RECEPTOR

被引：238
作者：
VITA, N
LAURENT, P
LEFORT, S
CHALON, P
DUMONT, X
KAGHAD, M
GULLY, D
LEFUR, G
FERRARA, P
CAPUT, D
机构：
[1] SANOFI ELF BIORECH,UNITE BIOL MOLEC GENE,BP 137,F-31676 LABEGE,FRANCE
[2] SANOFI RECH,F-31036 TOULOUSE,FRANCE
[3] SANOFI ELF BIORECH,UNITE BIOCHIM PROT,F-31676 LABEGE,FRANCE
来源：
FEBS LETTERS | 1993年 / 317卷 / 1-2期
关键词：
NEUROTENSIN; RECEPTOR; CLONING; BINDING; HT; 29; CELL;
D O I：
10.1016/0014-5793(93)81509-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A human neurotensin receptor (hNTR) cDNA was cloned from the colonic adenocarcinoma cell line HT29. The cloned cDNA encodes a putative peptide of 418 amino acids with 7 transmembrane domains. The amino acid sequence of the hNTR is 84% identical to the rat NTR [Neuron, 4 (1990) 847-854]. Transfection of this cDNA into COS cells results in the expression of receptors with pharmacological properties similar to those found with HT29 cells. Northern blot analysis using the hNTR cDNA probe indicated a single transcript of 4 kb in the brain, the small intestine and blood mononuclear cells.
引用
收藏
页码：139 / 142
页数：4
相关论文
共 21 条
[1]   REGULATION OF CYCLIC-GMP LEVELS BY NEUROTENSIN IN NEURO-BLASTOMA CLONE N1E115 [J].
AMAR, S ;
MAZELLA, J ;
CHECLER, F ;
KITABGI, P ;
VINCENT, JP .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1985, 129 (01) :117-125
[2]   PURIFICATION OF BIOLOGICALLY-ACTIVE GLOBIN MESSENGER-RNA BY CHROMATOGRAPHY ON OLIGOTHYMIDYLIC-ACID-CELLULOSE [J].
AVIV, H ;
LEDER, P .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1972, 69 (06) :1408-&
[3]   NEUROTENSIN STIMULATES INOSITOL TRISPHOSPHATE-MEDIATED CALCIUM MOBILIZATION BUT NOT PROTEIN KINASE-C ACTIVATION IN HT29 CELLS - INVOLVEMENT OF A G-PROTEIN [J].
BOZOU, JC ;
ROCHET, N ;
MAGNALDO, I ;
VINCENT, JP ;
KITABGI, P .
BIOCHEMICAL JOURNAL, 1989, 264 (03) :871-878
[4]   IDENTIFICATION OF A COMMON NUCLEOTIDE-SEQUENCE IN THE 3'-UNTRANSLATED REGION OF MESSENGER-RNA MOLECULES SPECIFYING INFLAMMATORY MEDIATORS [J].
CAPUT, D ;
BEUTLER, B ;
HARTOG, K ;
THAYER, R ;
BROWNSHIMER, S ;
CERAMI, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (06) :1670-1674
[5]  
CARRAWAY R, 1973, J BIOL CHEM, V248, P6854
[6]  
CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[7]  
GULLY D, 1992, IN PRESS P NATL ACAD
[8]   2 POPULATIONS OF NEUROTENSIN BINDING-SITES IN MURINE BRAIN - DISCRIMINATION BY THE ANTIHISTAMINE LEVOCABASTINE REVEALS MARKEDLY DIFFERENT AUTORADIOGRAPHIC DISTRIBUTION [J].
KITABGI, P ;
ROSTENE, W ;
DUSSAILLANT, M ;
SCHOTTE, A ;
LADURON, PM ;
VINCENT, JP .
EUROPEAN JOURNAL OF PHARMACOLOGY, 1987, 140 (03) :285-293
[9]  
KITABGI P, 1980, MOL PHARMACOL, V18, P11
[10]  
MAZELLA J, 1988, J BIOL CHEM, V263, P144
123