THE EFFECT OF INOCULATION WITH AZOTOBACTER AND AZOSPIRILLUM ON SOME MINERAL ACQUISITION, PHYTOHORMON AND GROWTH OF TOMATO SEEDLING

Sterilized soil was used to swon tomato seeds c.v. Hetof after inoculation with Azotobacter chroococcum and Azospirillum brasilense in single and combined inoculation. The results showed that the combined inoculation of both bacteria significantly increased the percentage of seed germination, dry weight, seedling vigor index, and the acquisition of NPK. Application of bacterial fertilizers influences the concentrations of IAA and ABA in leaves Moreover, seeds inculcation with both types of bacteria caused a greater increase in the total carbohydrate reduced sugar and soluble portions by (53.6%, 61.9% and 64.9%) compared with control treatment. From this results, it can be concluded that the combined inoculation of tomato seeds with both type of bacteria improving the nutrients status and the concentration of phytohormon, which reflecting on the growth of tomato seedlings and achieved tomato seedling for environmental stress.