STRUCTURE OF DOMAIN-1 OF RAT LYMPHOCYTE-T CD2 ANTIGEN

被引：147

作者：

DRISCOLL, PC ^{[1
]}

CYSTER, JG ^{[1
]}

CAMPBELL, ID ^{[1
]}

WILLIAMS, AF ^{[1
]}

机构：

[1] UNIV OXFORD, SIR WILLIAM DUNN SCH PATHOL, MRC, CELLULAR IMMUNOL RES UNIT, OXFORD OX1 3RE, ENGLAND

来源：

NATURE | 1991年 / 353卷 / 6346期

关键词：

D O I：

10.1038/353762a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

THE CD2 antigen is largely restricted to cells of the T-lymphocyte lineage and has been established as an important adhesion molecule in interactions between human T lymphocytes and accessory cells 1. In the adhesion reaction, CD2 on T cells binds to LFA-3 on other cells, with binding through domain 1 of CD2 (ref. 2). CD2 can also be a target for the delivery of mitogenic signals to T lymphocytes cultured with combinations of anti-CD2 antibodies 3,4. Two predictions that are contradictory have been made for the structure of CD2 domain 1. One suggests an immunoglobulin (Ig) fold, on the basis of sequence patterns conserved in the Ig-superfamily (IgSF) 5, whilst the other proposes a pattern of alternating alpha-helices and beta-strands, on the basis of secondary structure predictions 6. Thus CD2 domain 1 is an important test case for the validity of IgSF assignments based on sequence patterns. We report here the expression of domain 1 of rat CD2 in an Escherichia coli expression system and have determined a low-resolution solution structure by NMR spectroscopy.

引用

页码：762 / 765

页数：4

共 29 条

[1] 3-DIMENSIONAL STRUCTURE OF IMMUNOGLOBULINS [J].