ANALYSIS OF CYP1A1 PROMOTER FUNCTION BY TRANSCRIPTION INVITRO

被引：8
|
作者：
WEN, LP [1 ]
JONES, KW [1 ]
WHITLOCK, JP [1 ]
机构：
[1] STANFORD UNIV, MED CTR, SCH MED, DEPT PHARMACOL, STANFORD, CA 94305 USA
来源：
MOLECULAR CARCINOGENESIS | 1991年 / 4卷 / 02期
关键词：
CYTOCHROME-P450; GENE EXPRESSION;
D O I：
10.1002/mc.2940040203
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have analyzed the function of the CYP1A1 promoter using in vitro transcription. Using nuclear extracts from HeLa cells, we found that transcription from the promoter in vitro was constitutive. Transcription in vitro was not increased by prior exposure of the HeLa cells to the inducer 2,3,7,8-tetrachlorodibenzo-p-dioxin nor by the inclusion of a dioxin-responsive enhancer in the DNA template. Analyses of mutants revealed that a TATAAA sequence and two recognition motifs for CCAAT box transcription factor/nuclear factor l contributed to the constitutive activity of the promoter in vitro.
引用
收藏
页码：93 / 96
页数：4
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