QUATERNARY STRUCTURE OF URIDINE PHOSPHORYLASE FROM ESCHERICHIA-COLI K-12

被引：0
作者：
TSUPRUN, VL
TAGUNOVA, IV
LINKOVA, EV
MIRONOV, AS
机构：
来源：
BIOCHEMISTRY-MOSCOW | 1991年 / 56卷 / 05期
关键词：
URIDINE PHOSPHORYLASE; QUATERNARY STRUCTURE; ELECTRON MICROSCOPY;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The enzyme uridine phosphorylase was isolated from E. coli cells in homogeneous form. The molecular weight of the molecule, determine by gel filtration, approximately corresponds to a hexamer consisting of monomers with molecular weight 27.5 kD. The hexamer structure of uridine phosphorylase was directly demonstrated by electron microscopy, using digital image processing. It was found that in the enzyme molecule six monomers are arranged in two layers (three monomers in each layer) at the vertices of a triangular antiprism with point group symmetry 32.
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页码：640 / 643
页数：4
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