CLONING AND SEQUENCING OF A CDNA FOR THE DELTA-SUBUNIT OF PHOTOSYNTHETIC ATP-SYNTHASE (EC-3.6.1.34) FROM PEA (PISUM-SATIVUM)

被引：2

作者：

HOESCHE, JA ^{[1
]}

BERZBORN, RJ ^{[1
]}

机构：

[1] RUHR UNIV BOCHUM,DEPT BIOL,LEHRSTUHL BIOCHEM PFLANZEN,BOX 102148,W-4630 BOCHUM 1,GERMANY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1171卷 / 02期

关键词：

PHOTOPHOSPHORYLATION; RECONSTITUTION; ANTIBODY; (P-SATIVUM);

D O I：

10.1016/0167-4781(92)90121-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lambdagt10 cDNA clones for the nuclear encoded subunit delta of chloroplast ATP-synthase from Pisum sativum have been isolated. The 5' end was completed by PCR. The sequenced cDNA codes for the import precursor. N-Terminal sequencing of the mature protein isolated from chloroplasts revealed that the processing sites of the transit peptide from Pisum sativum and Spinacea oleracea are similar. The overall homology of the deduced amino acid sequences of the mature delta proteins from higher plants is about 40%. The conservation among hydrophilic residues is higher than for hydrophobic ones, indicating that the surface of delta is important for its function within the ATP-synthase.

引用

页码：201 / 204

页数：4

共 22 条

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