MOLECULAR-CLONING OF MULTIPLE SUBTYPES OF A NOVEL RAT-BRAIN ISOFORM OF THE ALPHA-1 SUBUNIT OF THE VOLTAGE-DEPENDENT CALCIUM-CHANNEL

被引：185
作者：
HUI, A
ELLINOR, PT
KRIZANOVA, O
WANG, JJ
DIEBOLD, RJ
SCHWARTZ, A
机构：
[1] Department of Pharmacology, Cell Biophysics University, Cincinnati College of Medicine Cincinnati
来源：
NEURON | 1991年 / 7卷 / 01期
关键词：
D O I：
10.1016/0896-6273(91)90072-8
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Several cDNAs encoding an isoform of the alpha-1 subunit of the voltage-dependent calcium channel were isolated from rat brain cDNA libraries. The complete nucleotide sequence of 6975 bp encodes a protein of 1634 amino acids, which corresponds to an M(r) of 186,968. The protein exhibits 71% and 76% homology to skeletal and cardiac alpha-1 subunits, respectively. When compared with skeletal and cardiac alpha-1 isoforms, the rat brain protein is intermediate in size at the amino terminus and shorter at the carboxyl terminus. Multiple subtypes of this alpha-1 isoform cDNA were characterized. These are indicative of alternative splicing of a primary transcript and encode three variants between motif I and motif II and two within the S3 region of motif IV. Thus, multiple isoforms of this rat brain alpha-1 subunit are possible.
引用
收藏
页码：35 / 44
页数：10
相关论文
共 47 条
[41]   RAT-BRAIN EXPRESSES A HETEROGENEOUS FAMILY OF CALCIUM CHANNELS [J].
SNUTCH, TP ;
LEONARD, JP ;
GILBERT, MM ;
LESTER, HA ;
DAVIDSON, N .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (09) :3391-3395
[42]  
STRIESSNIG J, 1990, J BIOL CHEM, V265, P363
[43]   PRIMARY STRUCTURE OF THE RECEPTOR FOR CALCIUM-CHANNEL BLOCKERS FROM SKELETAL-MUSCLE [J].
TANABE, T ;
TAKESHIMA, H ;
MIKAMI, A ;
FLOCKERZI, V ;
TAKAHASHI, H ;
KANGAWA, K ;
KOJIMA, M ;
MATSUO, H ;
HIROSE, T ;
NUMA, S .
NATURE, 1987, 328 (6128) :313-318
[44]   REGIONS OF THE SKELETAL-MUSCLE DIHYDROPYRIDINE RECEPTOR CRITICAL FOR EXCITATION CONTRACTION COUPLING [J].
TANABE, T ;
BEAM, KG ;
ADAMS, BA ;
NIIDOME, T ;
NUMA, S .
NATURE, 1990, 346 (6284) :567-569
[45]   RESOLUTION OF THE IDENTITY OF THE CA-2+-ANTAGONIST RECEPTOR IN SKELETAL-MUSCLE [J].
VAGHY, PL ;
MCKENNA, E ;
ITAGAKI, K ;
SCHWARTZ, A .
TRENDS IN PHARMACOLOGICAL SCIENCES, 1988, 9 (11) :398-402
[46]  
VAGHY PL, 1987, J BIOL CHEM, V262, P14337
[47]   ALTERATION AND RESTORATION OF K+ CHANNEL FUNCTION BY DELETIONS AT THE N-TERMINUS AND C-TERMINUS [J].
VANDONGEN, AMJ ;
FRECH, GC ;
DREWE, JA ;
JOHO, RH ;
BROWN, AM .
NEURON, 1990, 5 (04) :433-443
12345