MOLECULAR-CLONING OF MULTIPLE SUBTYPES OF A NOVEL RAT-BRAIN ISOFORM OF THE ALPHA-1 SUBUNIT OF THE VOLTAGE-DEPENDENT CALCIUM-CHANNEL

被引：184
作者：
HUI, A
ELLINOR, PT
KRIZANOVA, O
WANG, JJ
DIEBOLD, RJ
SCHWARTZ, A
机构：
[1] Department of Pharmacology, Cell Biophysics University, Cincinnati College of Medicine Cincinnati
来源：
NEURON | 1991年 / 7卷 / 01期
关键词：
D O I：
10.1016/0896-6273(91)90072-8
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Several cDNAs encoding an isoform of the alpha-1 subunit of the voltage-dependent calcium channel were isolated from rat brain cDNA libraries. The complete nucleotide sequence of 6975 bp encodes a protein of 1634 amino acids, which corresponds to an M(r) of 186,968. The protein exhibits 71% and 76% homology to skeletal and cardiac alpha-1 subunits, respectively. When compared with skeletal and cardiac alpha-1 isoforms, the rat brain protein is intermediate in size at the amino terminus and shorter at the carboxyl terminus. Multiple subtypes of this alpha-1 isoform cDNA were characterized. These are indicative of alternative splicing of a primary transcript and encode three variants between motif I and motif II and two within the S3 region of motif IV. Thus, multiple isoforms of this rat brain alpha-1 subunit are possible.
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页码：35 / 44
页数：10
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