BIOTINYL ANALOGS OF AMYLIN AS BIOLOGICALLY-ACTIVE PROBES FOR AMYLIN CGRP RECEPTOR RECOGNITION

被引：3
|
作者：
CHANTRY, A
FOOT, EA
LEIGHTON, B
DAY, AJ
WILLIS, AC
机构：
[1] MRC Immunochemistry Unit, University of Oxford, Oxford, OX1 3QU, South Parks Road
[2] Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, South Parks Road
来源：
FEBS LETTERS | 1992年 / 296卷 / 02期
关键词：
AMYLIN; CGRP; RECEPTOR; BIOTINYLATION; NON-INSULIN-DEPENDENT DIABETES-MELLITUS;
D O I：
10.1016/0014-5793(92)80362-K
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Biotinyl analogues of rat amylin were synthesised with sulfosuccinimidyl 2-(biotinamido) ethyl-1,3-dithiopropionate (NHS-SS-Biotin). Biotinylated amylin peptides were purified by HPLC, quantitated, and the presence of the biotin group at Lys-1 confirmed by peroxidase-labelled avidin and FAB mass spectroscopy. Amylin-biotin retained a similar affinity for binding to rat liver plasma membranes compared with rat amylin an a so completely inhibited insulin-stimulated glycogen synthesis in rat soleus muscle incubated in vitro. These biologically active amylin probes will enable a complete analysis of amylin/CGRP receptor expression in various cell types and facilitate the isolation and characterisation of the hormone-receptor complex.
引用
收藏
页码：123 / 127
页数：5
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