RESCUING TRANSGENE EXPRESSION BY COINTEGRATION

被引：70

作者：

CLARK, AJ ^{[1
]}

COWPER, A ^{[1
]}

WALLACE, R ^{[1
]}

WRIGHT, G ^{[1
]}

SIMONS, JP ^{[1
]}

机构：

[1] PHARMACEUT PROT LTD,CTR GENOME RES,EDINBURGH EH9 3JQ,SCOTLAND

来源：

BIO-TECHNOLOGY | 1992年 / 10卷 / 11期

关键词：

D O I：

10.1038/nbt1192-1450

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

To test whether foreign gene expression can be improved in transgenic mice by manipulating the site of integration, we co-integrated the efficiently expressed sheep beta-lactoglobulin gene with two poorly expressed beta-lactoglobulin-derived hybrid genes encoding human proteins. In each case, we observed a significant improvement in the frequency and level of expression of the hybrid gene. ''Rescuing'' transgene expression by co-integration may provide a general solution for improving the efficiency of heterologous gene expression in transgenic animals.

引用

页码：1450 / 1454

页数：5

共 20 条

[1] CHARACTERIZATION OF THE GENE ENCODING OVINE BETA-LACTOGLOBULIN - SIMILARITY TO THE GENES FOR RETINOL BINDING-PROTEIN AND OTHER SECRETORY PROTEINS [J].

ALI, S ;

CLARK, AJ .

JOURNAL OF MOLECULAR BIOLOGY, 1988, 199 (03) :415-426

[2] CHARACTERIZATION OF THE ALLELES ENCODING OVINE BETA-LACTOGLOBULIN-A AND BETA-LACTOGLOBULIN-B [J].

ALI, S ;

MCCLENAGHAN, M ;

SIMONS, JP ;

CLARK, AJ .

GENE, 1990, 91 (02) :201-207

[3] TRANSGENES AS PROBES FOR ACTIVE CHROMOSOMAL DOMAINS IN MOUSE DEVELOPMENT [J].

ALLEN, ND ;

CRAN, DG ;

BARTON, SC ;

HETTLE, S ;

REIK, W ;

SURANI, MA .

NATURE, 1988, 333 (6176) :852-855

[4] EXPRESSION OF A FOREIGN GENE IN A LINE OF TRANSGENIC MICE IS MODULATED BY A CHROMOSOMAL POSITION EFFECT [J].

ALSHAWI, R ;

KINNAIRD, J ;

BURKE, J ;

BISHOP, JO .

MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (03) :1192-1198

[5] EXPRESSION OF ACTIVE HUMAN CLOTTING FACTOR-IX FROM RECOMBINANT DNA CLONES IN MAMMALIAN-CELLS [J].

ANSON, DS ;

AUSTEN, DEG ;

BROWNLEE, GG .

NATURE, 1985, 315 (6021) :683-685

[6] HIGH-LEVEL EXPRESSION OF BIOLOGICALLY-ACTIVE HUMAN ALPHA-1-ANTITRYPSIN IN THE MILK OF TRANSGENIC MICE [J].

ARCHIBALD, AL ;

MCCLENAGHAN, M ;

HORNSEY, V ;

SIMONS, JP ;

CLARK, AJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (13) :5178-5182

[7] SYNTHESIS OF FUNCTIONAL HUMAN-HEMOGLOBIN IN TRANSGENIC MICE [J].

BEHRINGER, RR ;

RYAN, TM ;

REILLY, MP ;

ASAKURA, T ;

PALMITER, RD ;

BRINSTER, RL ;

TOWNES, TM .

SCIENCE, 1989, 245 (4921) :971-973

[8] PATTERNS OF EXPRESSION OF POSITION-DEPENDENT INTEGRATED TRANSGENES IN MOUSE EMBRYO [J].

BONNEROT, C ;

GRIMBER, G ;

BRIAND, P ;

NICOLAS, JF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (16) :6331-6335

[9] INTRONS INCREASE TRANSCRIPTIONAL EFFICIENCY IN TRANSGENIC MICE [J].

BRINSTER, RL ;

ALLEN, JM ;

BEHRINGER, RR ;

GELINAS, RE ;

PALMITER, RD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (03) :836-840

[10] CELL-SPECIFIC EXPRESSION OF A TRANSFECTED HUMAN ALPHA-1-ANTITRYPSIN GENE [J].

CILIBERTO, G ;

DENTE, L ;

CORTESE, R .

CELL, 1985, 41 (02) :531-540

← 1 2 →