CLONING AND MAPPING OF TELOMERE-ASSOCIATED SEQUENCES FROM HORDEUM-VULGARE L

被引：62

作者：

KILIAN, A

KLEINHOFS, A

机构：

[1] WASHINGTON STATE UNIV,DEPT GENET & CELL BIOL,PULLMAN,WA 99164

[2] SILESIAN UNIV,DEPT GENET,PL-40032 KATOWICE,POLAND

来源：

MOLECULAR & GENERAL GENETICS | 1992年 / 235卷 / 01期

关键词：

TELOMERES; CLONING; MAPPING; BARLEY; VECTORETTE PCR;

D O I：

10.1007/BF00286193

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a novel approach to the efficient cloning of telomere-associated sequences and demonstrate its application to the cloning and mapping of these sequences from barley. The method is a modification of the Vectorette PCR technique and allows specific amplification and cloning of subtelomeric sequences. Telomere-associated sequences isolated from barley include hypervariable, repetitive sequences. Polymorphisms detected by subtelomeric markers behaved as Mendelian factors and were mapped to the most distal positions of barley chromosomes 1, 2, and 4.

引用

页码：153 / 156

页数：4

共 25 条

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Arnold C, 1991, PCR Methods Appl, V1, P39

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