SIMPLE DERIVATION OF TFIID-DEPENDENT RNA POLYMERASE-II TRANSCRIPTION SYSTEMS FROM SCHIZOSACCHAROMYCES POMBE AND OTHER ORGANISMS, AND FACTORS REQUIRED FOR TRANSCRIPTIONAL ACTIVATION

被引：27
作者：
FLANAGAN, PM
KELLEHER, RJ
TSCHOCHNER, H
SAYRE, MH
KORNBERG, RD
机构：
[1] Department of Cell Biology, Fairchild Center, Stanford Univ. School of Medicine, Stanford
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 16期
关键词：
SACCHAROMYCES-CEREVISIAE; HUMAN T-CELLS; INVITRO TRANSCRIPTION SYSTEM; ACIDIC ACTIVATORS; TRANSCRIPTION FACTOR TFIID;
D O I：
10.1073/pnas.89.16.7659
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Resolution of whole cell extract through two chromatographic steps yields a single protein fraction requiring only the addition of TFIID for the initiation of transcription at RNA polymerase II promoters. This approach allows the convenient generation of RNA polymerase II transcription systems from Saccharomyces cerevisiae, human lymphocytes, and Schizosaccharomyces pombe. TFIIDs from all three organisms are interchangeable among all three systems. The S. cerevisiae and Sch. pombe systems support effects of acidic activator proteins, provided a further protein fraction from S. cerevisiae is supplied. This further fraction is distinct from the mediator of transcriptional activation described previously and represents a second component in addition to general initiation factors that may facilitate a response to acidic activators.
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页码：7659 / 7663
页数：5
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