ELUCIDATION OF CDNA SEQUENCES FOR METALLOTHIONEINS FROM RAINBOW-TROUT, STONE LOACH AND PIKE LIVER USING THE POLYMERASE CHAIN-REACTION

被引：53
作者：
KILLE, P
STEPHENS, PE
KAY, J
机构：
[1] UNIV WALES COLL CARDIFF,COLL CARDIFF,DEPT BIOCHEM,CARDIFF CF1 1ST,WALES
[2] CELLTECH LTD,SLOUGH,ENGLAND
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1089卷 / 03期
关键词：
FISH METALLOTHIONEIN; CDNA; POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY; 3' UNTRANSLATED VARIATION;
D O I：
10.1016/0167-4781(91)90187-Q
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Metallothionein cDNAs were generated from the livers of three fish species and amplified by PCR. Two distinct coding sequences (A and B) were elucidated for rainbow trout metallothioneins but single isoforms were encoded by genes isolated from the stone loach and pike. Different sized transcripts were observed both with stone loach and pike but these were accounted for by altered lengths of 3' untranslated regions.
引用
收藏
页码：407 / 410
页数：4
相关论文
共 16 条
[1]   THE RAINBOW-TROUT METALLOTHIONEINS - MOLECULAR-CLONING AND CHARACTERIZATION OF 2 DISTINCT CDNA SEQUENCES [J].
BONHAM, K ;
ZAFARULLAH, M ;
GEDAMU, L .
DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1987, 6 (06) :519-528
[2]   A COMPARISON OF THE DIFFERENTIAL ACCUMULATION OF CADMIUM IN THE TISSUES OF 3 SPECIES OF FRESH-WATER FISH, SALMO-GAIRDNERI, RUTILUS-RUTILUS AND NOEMACHEILUS-BARBATULUS [J].
BROWN, MW ;
THOMAS, DG ;
SHURBEN, D ;
SOLBE, JFDG ;
KAY, J ;
CRYER, A .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY, 1986, 84 (02) :213-217
[3]   SEQUESTRATION OF ENVIRONMENTAL CADMIUM BY METALLOTHIONEIN IN THE ROACH (RUTILUS-RUTILUS) AND THE STONE LOACH (NOEMACHEILUS-BARBATULUS) [J].
BROWN, MW ;
SHURBEN, D ;
SOLBE, JFDG ;
CRYER, A ;
KAY, J .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, 1987, 87 (01) :65-69
[4]   MOLECULAR-CLONING OF METALLOTHIONEIN CDNA AND ANALYSIS OF METALLOTHIONEIN GENE-EXPRESSION IN WINTER FLOUNDER TISSUES [J].
CHAN, KM ;
DAVIDSON, WS ;
HEW, CL ;
FLETCHER, GL .
CANADIAN JOURNAL OF ZOOLOGY-REVUE CANADIENNE DE ZOOLOGIE, 1989, 67 (10) :2520-2527
[5]   SYNTHESIS OF CALF PROCHYMOSIN (PRORENNIN) IN ESCHERICHIA-COLI [J].
EMTAGE, JS ;
ANGAL, S ;
DOEL, MT ;
HARRIS, TJR ;
JENKINS, B ;
LILLEY, G ;
LOWE, PA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (12) :3671-3675
[6]  
GEORGE S, 1990, MAR ENVIRON RES, V28, P173
[7]   BIOCHEMISTRY OF METALLOTHIONEIN [J].
KAGI, JHR ;
SCHAFFER, A .
BIOCHEMISTRY, 1988, 27 (23) :8509-8515
[8]   N-TERMINAL SEQUENCE OF METALLOTHIONEIN FROM RAINBOW-TROUT [J].
KAY, J ;
CRYER, A ;
BROWN, MW ;
NOREY, CG ;
BREMNER, I ;
OVERNELL, J ;
PARTEN, B ;
DUNN, BM .
BIOCHEMICAL SOCIETY TRANSACTIONS, 1987, 15 (03) :453-454
[9]   CADMIUM ACCUMULATION AND PROTEIN-BINDING PATTERNS IN TISSUES OF THE RAINBOW-TROUT, SALMO-GAIRDNERI [J].
KAY, J ;
THOMAS, DG ;
BROWN, MW ;
CRYER, A ;
SHURBEN, D ;
SOLBE, JFD ;
GARVEY, JS .
ENVIRONMENTAL HEALTH PERSPECTIVES, 1986, 65 :133-139
[10]   NATURALLY-OCCURRING AND RECOMBINANT METALLOTHIONEINS - STRUCTURE, IMMUNOREACTIVITY AND METAL-BINDING FUNCTIONS [J].
KAY, J ;
CRYER, A ;
DARKE, BM ;
KILLE, P ;
LEES, WE ;
NOREY, CG ;
STARK, JM .
INTERNATIONAL JOURNAL OF BIOCHEMISTRY, 1991, 23 (01) :1-5
12