FULLY AUTOMATED AMINO-ACID-ANALYSIS FOR PROTEIN AND PEPTIDE HYDROLYSATES BY PRECOLUMN DERIVATIZATION WITH 9-FLUORENYL METHYLCHLOROFORMATE AND 1-AMINOADAMANTANE

被引：70

作者：

GUSTAVSSON, B ^{[1
]}

BETNER, I ^{[1
]}

机构：

[1] PHARM LKB BIOTECHNOL,BOX 305,S-16126 BROMMA,SWEDEN

来源：

JOURNAL OF CHROMATOGRAPHY | 1990年 / 507卷

关键词：

D O I：

10.1016/S0021-9673(01)84182-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A fully automated precolumn dericatization method for the determination of primary and secondary amino acids using reversed-phase chromatography is described. The derivatization reagent 9-fluorenyl methylchloroformate is used, together with 1-aminoadamantane for reaction with excess of reagent. The derivatization procedure is described in detail. Reproducibility data are presented and compared between the manual and the fully automated methods. Optimization strategies for separation, and differences in detection between fluorescence and UV methods are discussed. Analytical data for protein and peptide hydrolysates are compared with their sequenced data. © 1990.

引用

页码：67 / 77

页数：11

共 7 条

[1]

BETNER I, 1988, LC GC-MAG SEP SCI, V6, P832

[2] NEW AUTOMATED AMINO-ACID-ANALYSIS BY HPLC PRECOLUMNS DERIVATIZATION WITH FLUORENYLMETHYLOXYCARBONYLCHLORIDE [J].