THE HUMAN CYTOCHROME B(5), GENE AND 2 OF ITS PSEUDOGENES ARE LOCATED ON CHROMOSOMES 18Q23, 14Q31-32.1 AND 20P11.2, RESPECTIVELY

被引：8

作者：

GIORDANO, SJ

YOO, M

WARD, DC

BHATT, M

OVERHAUSER, J

STEGGLES, AW

机构：

[1] Northeastern Ohio Univ Coll Med & Pharm, DEPT BIOCHEM, ROOTSTOWN, OH 44272 USA

[2] KEIMYUNG UNIV, DEPT BIOL, TAEGU, SOUTH KOREA

[3] YALE UNIV, DEPT MED GENET, NEW HAVEN, CT 06510 USA

[4] THOMAS JEFFERSON UNIV, JEFFERSON MED COLL, DEPT BIOCHEM & MOLEC BIOL, PHILADELPHIA, PA 19107 USA

来源：

HUMAN GENETICS | 1993年 / 92卷 / 06期

关键词：

D O I：

10.1007/BF00420948

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Using very high stringency hybridization conditions for the Southern blot hybridization analysis of hamster-human cell hybrid DNA, we were able to map the human cytochrome b(5) gene and two of its pseudogenes (psgb(5)1 and psgb(5)2) unambiguously to chromosomes 18, 14, and 20. These localizations were confirmed and extended to 18q23, 14q31-32.1, and 20p11.2 by using a combination of nonisotopic in situ hybridization of chromosomal spreads and the polymerase chain reaction analysis of DNA samples isolated from somatic cell hybrids retaining deletions or translocations of chromosome 18.

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页码：615 / 618

页数：4

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