DETECTION OF HUMAN CYTOMEGALOVIRUS DNA IN PARAFFIN SECTIONS OF HUMAN BRAIN BY POLYMERASE CHAIN-REACTION AND THE OCCURRENCE OF FALSE NEGATIVE RESULTS

被引：15

作者：

GASS, P

KIESSLING, M

SCHAFER, P

MESTER, C

SCHMITT, HP

KUHN, JE

机构：

[1] UNIV HEIDELBERG,INST VIROL,W-6900 HEIDELBERG,GERMANY

[2] UNIV COLOGNE,INST VIROL,W-5000 COLOGNE 41,GERMANY

来源：

JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY | 1993年 / 56卷 / 02期

关键词：

D O I：

10.1136/jnnp.56.2.211

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Paraffin-embedded necropsy material from 6 patients with human cytomegalovirus encephalitis (HCMVE) corroborated by immunocytochemistry and 11 control cases were examined for the presence of human cytomegalovirus (HCMV) DNA by a nested polymerase chain reaction (nPCR). A characteristic 183 base pair (bp) fragment of the HCMV genome could readily be amplified in 4 cases of HCMVE. In 2 cases of HCMVE, viral DNA could be demonstrated only sporadically by PCR, due most likely to inefficient DNA extraction or DNA degradation. All control cases remained negative. The nPCR provides a specific method for detecting HCMV DNA in routinely processed biopsy and necropsy material and may be used in archival tissues for the diagnosis of infection. Fixation of samples and DNA extraction are, however, crucial steps and require careful control if PCR is used for detection of HCMV, to avoid false negative results.

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页码：211 / 214

页数：4

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