FLUORIDE IS A STRONG AND SPECIFIC INHIBITOR OF (ASYMMETRICAL) AP4A HYDROLASES

被引：43
作者：
GURANOWSKI, A
机构：
[1] Katedra Biochemii, Akademia Rolnicza, ul. Wolyńska 35
来源：
FEBS LETTERS | 1990年 / 262卷 / 02期
关键词：
Ap[!sub]4[!/sub]A hydrolase; Enzyme inhibition; Fluoride;
D O I：
10.1016/0014-5793(90)80190-T
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Fluoride acts as a noncompetitive, strong inhibitor of (asymmetrical) Ap4A hydrolases (EC 3.6.1.17). The Ki values estimated for the enzymes isolated from seeds of some higher plants (yellow lupin, sunflower and marrow) are in the range of 2-3 μM and I50 for the hydrolase from a mammalian tissue (beef liver) is 20 μM. The anion, up to 25 mM, does not affect the following other enzymes which are able to degrade the bis(5'-nucleosidyl)-oligophosphates: Escherichia coli (symmetrical) Ap4A hydrolase (EC 3.6.1.41), yeast Ap4A phosphorylase (EC 2.7.7.53), yellow lupin Ap3A hydrolase (EC 3.6.1.29) and phosphodiesterase (EC 3.1.4.1). None of halogenic anions but fluoride affects the activity of (asymmetrical) Ap4A hydrolases. Usefulness of the fluoride effect for the in vivo studies on the Ap4A metabolism is shortly discussed. © 1990.
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页码：205 / 208
页数：4
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