5-METHYLCYTOSINE CONTENT OF DNA DURING DIFFERENTIATION IN MOUSE TERATOCARCINOMA CELLS

被引：10

作者：

FABRICANT, JD ^{[1
]}

WAGNER, EF ^{[1
]}

AUER, B ^{[1
]}

SCHWEIGER, M ^{[1
]}

机构：

[1] INST PASTEUR, DEPT BIOL MOLEC, F-75015 PARIS, FRANCE

来源：

EXPERIMENTAL CELL RESEARCH | 1979年 / 124卷 / 01期

关键词：

D O I：

10.1016/0014-4827(79)90253-2

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

DNA of mouse teratocarcinoma cells has been analysed as to content of methylated bases by a sensitive method based on two consecutive steps of two-dimensional thin-layer chromatography of radioactively labelled DNA bases. In pluripotent embryonal carcinoma cells (EC), and EC cells cultured under differentiating conditions, as well as teratoma-derived myoblasts and fibroblasts, 5-methylcytosine (5-MC) was the only methylated base found. DNA of the differentiated cell lines (fibroblasts and myoblasts) contained 3.3% and 3.6% 5-MC respectively, while that of embryonal carcinoma cells had 3.8%-5.2%, depending on the cell line. During in vitro differentiation the PCC3/A/1 cell line showed some decrease in percentage of 5-MC (4.2% for EC cells and 3.8% for 30-day cultures). © 1979.

引用

页码：25 / 29

页数：5

共 25 条

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