5-METHYLCYTOSINE CONTENT OF DNA DURING DIFFERENTIATION IN MOUSE TERATOCARCINOMA CELLS

被引:10
作者
FABRICANT, JD [1 ]
WAGNER, EF [1 ]
AUER, B [1 ]
SCHWEIGER, M [1 ]
机构
[1] INST PASTEUR, DEPT BIOL MOLEC, F-75015 PARIS, FRANCE
关键词
D O I
10.1016/0014-4827(79)90253-2
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
DNA of mouse teratocarcinoma cells has been analysed as to content of methylated bases by a sensitive method based on two consecutive steps of two-dimensional thin-layer chromatography of radioactively labelled DNA bases. In pluripotent embryonal carcinoma cells (EC), and EC cells cultured under differentiating conditions, as well as teratoma-derived myoblasts and fibroblasts, 5-methylcytosine (5-MC) was the only methylated base found. DNA of the differentiated cell lines (fibroblasts and myoblasts) contained 3.3% and 3.6% 5-MC respectively, while that of embryonal carcinoma cells had 3.8%-5.2%, depending on the cell line. During in vitro differentiation the PCC3/A/1 cell line showed some decrease in percentage of 5-MC (4.2% for EC cells and 3.8% for 30-day cultures). © 1979.
引用
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页码:25 / 29
页数:5
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