PRODUCTION AND CHARACTERIZATION OF OVINE GM-CSF EXPRESSED IN MAMMALIAN AND BACTERIAL-CELLS

被引：2

作者：

OBRIEN, PM

SEOW, HF

ENTRICAN, G

COUPAR, BEH

WOOD, PR

机构：

[1] CSIRO,DIV ANIM HLTH,PARKVILLE,VIC 3052,AUSTRALIA

[2] MOREDUN RES INST,EDINBURGH EH17 7JH,MIDLOTHIAN,SCOTLAND

[3] CSIRO,AUSTRALIAN ANIM HLTH LAB,GEELONG,VIC 3220,AUSTRALIA

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 1995年 / 48卷 / 3-4期

关键词：

SHEEP; GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR; BONE MARROW; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI;

D O I：

10.1016/0165-2427(95)05433-7

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A cDNA encoding ovine granulocyte-macrophage colony-stimulating factor (GM-CSF) was isolated and two forms of recombinant ovine GM-CSF were produced. A glycosylated form was produced in mammalian cells infected with a recombinant vaccinia virus encoding ovine GM-CSF. Recombinant ovine GM-CSF was also produced in Escherichia coli and purified by affinity chromatography. Both forms of the protein were detected by ovine GM-CSF-specific monoclonal antibodies, and exhibited activity on ovine bone marrow haemopoetic progenitor cells.

引用

页码：287 / 298

页数：12

共 37 条

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