AN IMPROVED METHOD FOR THE PREPARATION OF THE PHOSPHORAMIDITES OF MODIFIED 2'-DEOXYNUCLEOTIDES - INCORPORATION OF 8-OXO-2'-DEOXY-7H-GUANOSINE INTO SYNTHETIC OLIGOMERS

被引：55
作者：
BODEPUDI, V
IDEN, CR
JOHNSON, F
机构：
[1] Department of Pharmacological Sciences, School of Medicine, Health Sciences Center, State University of New York at Stony Brook, Stony Brook
来源：
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 1991年 / 10卷 / 04期
基金：
美国国家卫生研究院;
关键词：
D O I：
10.1080/07328319108046660
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
3'-O-(Diisopropylamino-2-cyanoethoxyphosphinyl)-5'-O-(4,4'-dimethoxytrityl)-N2-isobutyryl-8-oxo-2'-deoxy-7H-guanosine (7) was synthesized and used for the introduction of an 8-oxo-2'-deoxy-7H-guanosine residue into a DNA oligomer by means of automated synthesis. A modification of the preparation of the phosphoramidite markedly improves the coupling efficiency in the oligomer synthesis in this and several other cases that were tested.
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收藏
页码：755 / 761
页数：7
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