AN IMPROVED ASSAY FOR LONG-CHAIN ACYL-COA SYNTHETASE

被引：14

作者：

GALTON, DJ

FRASER, TR

机构：

[1] Department of Medicine, Royal Postgraduate Medical School, London W.12, England

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 28卷 / 1-3期

关键词：

D O I：

10.1016/0003-2697(69)90156-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new and simple paper disc assay is described for measuring the activity of long-chain fatty acyl-CoA synthetases that depends on measuring the direct incorporation of 14C-palmitate into its product. Oleate was found to be unsatisfactory for this procedure due to difficulty of elution from paper discs. The behavior of the assay system is shown to be consistent with the expected properties of the enzyme, and this method has been found particularly useful for kinetic studies in our laboratory. © 1969.

引用

页码：59 / &