GENETIC DIVERSITY IN THE NONSTRUCTURAL GENE OF PARVOVIRUS B19 DETECTED BY SINGLE-STRANDED CONFORMATIONAL POLYMORPHISM ASSAY (SSCP) AND PARTIAL NUCLEOTIDE SEQUENCING

被引:32
作者
KERR, JR
CURRAN, MD
MOORE, JE
ERDMAN, DD
COYLE, PV
NUNOUE, T
MIDDLETON, D
FERGUSON, WP
机构
[1] ROYAL VICTORIA HOSP,REG VIRUS LAB,BELFAST BT12 6BA,ANTRIM,NORTH IRELAND
[2] BELFAST CITY HOSP,TISSUE TYPING LAB,BELFAST BT9 7AD,ANTRIM,NORTH IRELAND
[3] CTR DIS CONTROL & PREVENT,NATL CTR INFECT DIS,DIV VIRAL & RICKETTSIAL DIS,ATLANTA,GA 30341
[4] KYUSHU UNIV,SCH HLTH SCI,FUKUOKA 812,JAPAN
关键词
PARVOVIRUS B19; PCR; SINGLE-STRANDED CONFORMATIONAL POLYMORPHISM ASSAY (SSCP); NUCLEOTIDE SEQUENCING;
D O I
10.1016/0166-0934(95)00017-O
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A homologous region in the parvovirus B19 non-structural gene (B19 nt 1399-1682) was examined in 50 samples from patients with a wide variety of B19-related disease from various countries by PCR amplification, single-stranded conformational polymorphism (SSCP) assay and nucleotide sequencing, Five SSCP types were confirmed by nucleotide sequence analysis. Of a total of 6 mutations, all were silent. Types 3 and 4 accounted for 92% of strains. There was no correlation between genome type and either clinical illness or patient age. However, there was a correlation between SSCP type and country of origin. Type 3 strains predominated in Japan (18/26) and the UK (6/8), whereas type 4 predominated in the USA (9/12). Notably, type 3 strains also predominated among females (14/18), whereas there were approximately equal numbers of strain types 3 (7/17) and 4 (8/17) among males; an observation which remains unexplained. Within the Japanese group, although type 3 strains predominated overall, strains isolated from 1981 to 1987 consisted of types 1 (2/15), 2 (1/15), 3 (8/15), and 4 (4/15), whereas strains isolated from 1990 to 1994 consisted almost entirely of type 3 (10/11).
引用
收藏
页码:213 / 222
页数:10
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