MOUSE TESTIS PDHA-2 PROMOTER UPSTREAM SEQUENCES CONFER TISSUE-SPECIFIC AND TEMPORAL-SPECIFIC ACTIVITY IN TRANSGENIC MICE

被引：7

作者：

IANNELLO, RC

YOUNG, JC

SUMARSONO, S

TYMMS, MJ

KOLA, I

机构：

[1] Centre for Early Human Development, Institute for Reproduction and Development, Monash Medical Centre, Clayton

来源：

REPRODUCTION FERTILITY AND DEVELOPMENT | 1994年 / 6卷 / 05期

关键词：

SPERMATOGENESIS; TRANSCRIPTION FACTORS; MOUSE;

D O I：

10.1071/RD9940599

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Spermatogenesis is a complex process requiring the coordinate expression of a number of testis-specific genes. One of these, Pdha-2, codes for the murine testis-specific isoform of the Elm subunit of the pyruvate dehydrogenase complex. To elucidate the mechanisms regulating its expression in vivo, we have begun to investigate the Pdha-2 promoter in transgenic mice. In this paper, a construct containing 3.0 kb of promoter and upstream sequences is reported to be sufficient for directing the testis-specific expression of a CAT reporter gene in mice harbouring the transgene. Similarly to the endogenous Pdha-2, the CAT gene is expressed in testis in a stage-specific manner. However, the 3.0-kb Pdha-2 promoter is not active in somatic tissue suggesting that repressor elements may be present within these sequences.

引用

页码：599 / 604

页数：6

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