TRANSCRIPTION FROM A TATA-LESS PROMOTER REQUIRES A MULTISUBUNIT TFIID COMPLEX

被引：552

作者：

PUGH, BF ^{[1
]}

TJIAN, R ^{[1
]}

机构：

[1] UNIV CALIF BERKELEY,HOWARD HUGHES MED INST,DEPT MOLEC & CELL BIOL,BERKELEY,CA 94720

来源：

GENES & DEVELOPMENT | 1991年 / 5卷 / 11期

关键词：

TATA BOX-BINDING PROTEIN; TRANSCRIPTION; TFIID COMPLEX; TATA-INDEPENDENT; TBP;

D O I：

10.1101/gad.5.11.1935

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In eukaryotes, the TATA box-binding protein (TBP) is responsible for nucleating assembly of the transcription initiation machinery. Here, we report that a TFIID complex containing TBP is essential for transcription even at a promoter that lacks a TATA box. Immunopurification of TFIID reveals that the active species in reconstituting TATA-less transcription is a multisubunit complex consisting of TBP and many TBP-associated factors (TAFs).

引用

页码：1935 / 1945

页数：11

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