PURIFICATION OF OIDIODENDRON-KALRAI PROTEASES

被引：4
作者：
CINO, PM
TEWARI, RP
机构：
[1] RUTGERS STATE UNIV, DEPT MICROBIOL, NEW BRUNSWICK, NJ 08903 USA
[2] WAKSMAN INST MICROBIOL, NEW BRUNSWICK, NJ USA
来源：
CANADIAN JOURNAL OF MICROBIOLOGY | 1976年 / 22卷 / 03期
关键词：
D O I：
10.1139/m76-049
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
O. kalrai yeast-phase cells demonstrate proteolytic activity. Some of the proteolytic enzymes of the crude extract were purified by a combination of ammonium sulfate precipitation, Sephadex G-200 and DEAE cellulose column chromatography. At least 6 proteins exhibiting a range of proteolytic activities could be identified by these procedures. Purity of the enzyme fractions obtained from the DEAE-cellulose columns was tested by running polyacrylamide gels.
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收藏
页码：327 / 333
页数：7
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